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| Registros recuperados: 101 | |
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| Benjamin,Sailas; Pandey,Ashok. |
| Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40°C and pH 7-8.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200016 |
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| Benjamin,Sailas; Pandey,Ashok. |
| Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40ºC and pH 7-8.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500002 |
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| Pereira,C.G.; Saraiva,G.L.; Santos,M.R.; Assao,V.S.; Fietto,J.L.R.; Bressan,G.C.; Almeida,M.R.; Moreira,M.A.S.; Silva Júnior,A.. |
| RESUMO O vírus da bronquite infecciosa (IBV) é um importante patógeno respiratório presente na avicultura comercial e tem provocado grandes perdas econômicas em todo o mundo. A vacinação é realizada pela indústria produtora de aves, mas continuam surgindo novos sorotipos e variações antigênicas, dificultando o controle de IBV. Nós realizamos uma caracterização molecular de uma cepa de IBV obtida diretamente de tecidos e comparamos com a mesma cepa que havia sido passada três vezes em ovo embrionado. Nós mostramos uma variação significante na sequência viral depois de ter sido isolada em ovo embrionado. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Infectious bronchitis virus; Isolation; Characterization; Stability. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401333 |
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| Dufourcq, R.; Chalkiadakis, Eletherios; Fauchon, M.; Deslandes, Eric; Kerjean, V.; Chanteau, S.; Petit, Elodie; Guezennec, Jean; Dupont-rouzeyrol, M.. |
| Marine bacteria are a rich source of bioactive metabolites. However, the microbial diversity of marine ecosystem still needs to be explored. The aim of this study was to isolate and characterize bacteria with antimicrobial activities from various marine coastal environment of New Caledonia. We obtained 493 marine isolates from various environments and samples of which 63 (12.8%) presented an antibacterial activity against a panel of reference pathogenic strains (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis). Ten out of the most promising strains were cultured, fractionated and screened for antibacterial activity. Four of them (NC282, NC412, NC272 and NC120) showed at least an activity against reference and... |
| Tipo: Text |
Palavras-chave: Antibacterial activity; Characterization; Marine bacteria; New Caledonia; Screening. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00174/28552/26994.pdf |
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| Higuti,lma Hiroko; Grande,Simone Wichert; Sacco,Roberta; Nascimento,Aguinaldo José do. |
| One hundred and twenty five soil samples were collected from the regions of roots of corn, cassava, potato, bean, sugar cane, soya, and pumpkin. From these, 75 strains were isolated that produced a yellowish halo surrounding the colonies, due to a phenolphtalein-cyclodextrin (CD) complex, and these were selected as alkalophilic CGTase-producing bacteria. All the 75 strains were identified as Bacillus firmus by microscopy and biochemical tests. The activity of the CGTase's varied from 2² to 2(10) dilutions,when assayed by CD-trichloroethylene (TCE)-complex precipitation. Strain 31 that produced the enzyme at the higher level was selected, and its enzyme was partially purified by starch adsorption (x 17) in a yield of 51%. Maximum enzyme activity occurred at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Characterization. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200007 |
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| Duarte,Ana Rodrigues; Duarte,Débora Maria Rodrigues; Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Lima-Filho,José Luiz de; Porto,Ana Lúcia Figueiredo. |
| The partial characterization and purification of milk clotting enzyme obtained from the (root latex) of Jacaratia corumbensis O. kuntze was studied, by fractional precipitation with ammonium sulphate and ion exchange chromatography. The ammonium sulphate precipitate showed five fractions (AS1- 0-20%; AS2 - 20-40%; AS3 - 40-60%; AS4 - 60-80%; AS5 - 80-100%) and among the fractions obtained, the 40-60% fraction (AS3) showed the highest milk clotting activity with a purification factor of 1.2 fold in relation to the crude extract. This fraction when applied on Mono Q column yielded two protein peaks (p1 and p2), but p1 pool showed the best milk-clotting activity. The optimal pH for the crude and partially purified extract was 6.5 and 7.0, respectively. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Jacaratia corumbensis O. kuntze; Milk clotting enzyme; Characterization; Purification; Vegetable enzyme. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100001 |
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| Mazza, Mattia; Alliot, Cyrille; Sinquin, Corinne; Colliec-jouault, Sylvia; Reiller, Pascal E.; Huclier-markai, Sandrine. |
| Background: Exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium, showed anti-metastatic properties. They may represent a new class of ligands to be combined with theranostic radionuclides, such as 47Sc/44Sc. The goal of this work was to investigate the feasibility of such coupling. (2) Methods: EPSs, as well as heparin used as a drug reference, were characterized in terms of molar mass and dispersity using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering (AF4-MALS). The intrinsic viscosity of EPSs at different ionic strengths were measured in order to establish the conformation. To determine the stability constants of Sc with EPS and heparin, a Free-ion selective radiotracer extraction (FISRE)... |
| Tipo: Text |
Palavras-chave: Exopolysaccharides; Scandium; Theranostic; Characterization; Complexation. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00681/79332/81844.pdf |
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| BELLÉ,C.; KASPARY,T.E.; BALARDIN,R.R.; RAMOS,R.F.; ANTONIOLLI,Z.I.. |
| ABSTRACT: The frequency of species of root-knot nematodes (Meloidogyne spp.) was evaluated in weeds collected in different fallow farms in the State of Rio Grande do Sul, Brazil. In the samples where the nematode was found, the species of the root-knot nematode was identified by electrophoresis using the isozyme esterase. They were obtained from weeds belonging to 24 weed species from 13 different botanical families: Amaranthaceae, Asteraceae, Commelinaceae, Convovulaceae, Cyperaceae, Euphorbiaceae, Lamiaceae, Malvaceae, Oxalidaceae, Poaceae, Portulacaceae, Solanaceae, Verbenaceae. Meloidogyne javanica Est J3 (Rm: 1.0, 1.25, 1.40) was the most frequent species and occurred in 53.3% of the samples. M. arenaria with phenotype Est. A2 (Rm: 1.20, 1.30) was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Root-knot nematode; Weed plants; Isozyme esterase; Characterization; Hostability. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100292 |
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| Rodrigues,Plínio Ribeiro; Silvério,Tatiane Aparecida Barroso; Druzian,Janice Izabel. |
| Abstract Polymers are materials of pronounced importance in the modern world, since they are massively present in everyday life, especially in the form of packaging. However, most of these materials are derived from non-renewable sources and their disposal generates large volumes of waste, which is extremely damaging to natural environments. In this context, microbial biopolymers appear as a powerful alternative in the substitution of several applications of synthetic plastics, causing less harm to the environment, as they are biodegradable and produced from renewable sources. In this study, evaluation and characterization of polyhydroxyalkanoates (PHAs) produced by Cupriavidus necator (IPT 026 and IPT 027) and Burkholderia cepacia (IPT 119 and IPT 400),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crude glycerol; Biosynthesis; Polyhydroxyalkanoates; Characterization. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100209 |
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| Angelini,Michelle; Stehling,Eliana Guedes; Moretti,Maria Luiza; Silveira,Wanderley Dias da. |
| Shigella spp., the human pathogen responsible for shigellosis, is highly infectious even at low levels. The incidence rate of shigellosis varies with geographical distribution, location human development index, and age groups, being higher among children aged under 5 years. In Brazil, a few works indicate that shigellosis cases are underestimated, with S. flexneri and S. sonnei strains being the major agents responsible for the shigellosis cases. The present study used pulsed field gel electrophoresis (PFGE) to investigate the molecular epidemiology of 119 strains of S. sonnei and S. flexneri isolated from shigellosis cases that occurred in the metropolitan areas of Ribeirão Preto and Campinas Cities, São Paulo Sate, southeast Brazil. The results indicated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shigella sonnei; Shigella flexneri; Epidemiology; Characterization; PFGE. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300034 |
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| Ibarra Estrada, Martha Elva. |
| A México se le considera centro de origen del zapote mamey ya que cuenta con una gran diversidad. Sin embargo, son pocos los trabajos enfocados en estudiar tal diversidad tanto en la parte morfológica, contenido de ácidos graso o aspectos moleculares. Por lo anterior, el objetivo del presente trabajo fue: caracterizar morfológica, bioquímica y molecularmente selecciones sobresalientes de zapote mamey. En base a las variables de fruto y el perfil de ácidos grasos en semilla, el material vegetal estudiado mostró amplia variación entre las accesiones. El peso y longitud de fruto, longitud de hilio y de semilla, número de fibras, grosor de epicarpio y ancho de semilla fueron las variables determinantes para la agrupación de las selecciones. El perfil de... |
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Palavras-chave: Caracterización; Frutos; Variabilidad; RAPD; RAMP; Characterization; Fruits; Lipids; Seeds; Variability; Lípidos; Semillas; Doctorado; Fruticultura. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/740 |
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| FIGUEIRA,Luanda de Paula; SOARES,Fabiane Veloso; JÚNIOR,Roberto Daibes NAIFF; VINHOTE-SILVA,Alana Cristina; SILVA,Susi Simas da; ESPIR,Thaís Tibery; NAIFF,Maricleide de Farias; GOMES,Luis Henrique Monteiro; MOREIRA,Flávia Regina Almeida Campos Naief; FRANCO,Antonia Maria Ramos. |
| ABSTRACT Few cases of human cutaneous leishmaniasis (CL) caused by Leishmania naiffi were described in the medical literature. The aim of this study was to report and analyze new cases of L. naiffi in the period between the years 1992 to 2011. The strains were characterized by isoenzyme analysis. All patients assisted had small lesions; ranging from 1.0x1.0 mm and 13.5x11.5 mm. The lesions observed were widely distributed: 55.5% on the lower limb, 5.5% in the abdominal area, 16.6% on upper limb and 22.2% in upper limb and back. Seventy-two percent of patients had ulcerated lesions. Clinical course of the disease varied from 1 to 10 months. According to gender, most infected individuals were men (83.3%). The patients came from Amazonas (10), Pará (01) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: American Tegumentary Leishmaniasis; Leishmania naiffi; Characterization; Experimental stations; Lutzomyia. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000100047 |
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| Gomaa,Eman Zakaria. |
| The potentiality of 23 bacterial isolates to produce alkaline protease and carboxymethyl-cellulase (CMCase) on Ficus nitida wastes was investigated. Bacillus pumillus ATCC7061 was selected as the most potent bacterial strain for the production of both enzymes. It was found that the optimum production of protease and CMCase were recorded at 30 °C, 5% Ficus nitida leaves and incubation period of 72 h. The best nitrogen sources for protease and CMCase production were yeast extract and casein, respectively. Also maximum protease and CMCase production were reported at pH 9 and pH 10, respectively. The enzymes possessed a good stability over a pH range of 8-10, expressed their maximum activities at pH10 and temperature range of 30-50 °C, expressed their maximum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alkaliphillic; Protease; Carboxymethylcellulase; Production; Characterization. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200033 |
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| Peixoto, Ferdyanne Beatriz Sabóia; Peixoto, Jean Charles da Cunha; Assunção, Enedina Nogueira de; Peixoto, Elise Miranda; Pereira, José Odair; Astolfi-Filho, Spartaco. |
| Serratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP - redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbiologia 2.12.00.00-9 biodegradation; Characterization; DCPIP; Antimicrobial resistance. Microbiologia. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223 |
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| Correa, Cynthia Candida; Alves, Alexandre Florindo. |
| O uso dos produtos à base de plantas medicinais surge como uma forte tendência mundial. Esse é considerado um mercado promissor e apresenta números expressivos, tornando-se um ambiente de negócio atrativo. Entretanto, apesar de toda importância social e econômica, o Brasil e o Paraná não tem investido adequadamente na produção, industrialização e distribuição dessas plantas. Nesse contexto, buscou-se, através desse trabalho, caracterizar e analisar a produção de plantas medicinais no estado do Paraná. Para alcance desse objetivo foram utilizados dados secundários, encontrados em livros, artigos científicos, revistas e, principalmente, em sites governamentais. Pode-se concluir, a partir da pesquisa, que existe um aumento da oferta e demanda de plantas... |
| Tipo: Conference Paper or Presentation |
Palavras-chave: Plantas medicinais; Sistema produtivo; Caracterização; Paraná; Medicinal plants; Productive system; Characterization; Paraná state - Brazil; Health Economics and Policy. |
| Ano: 2008 |
URL: http://purl.umn.edu/108079 |
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| Xiao,Anfeng; Xu,Caiyun; Lin,Yan; Ni,Hui; Zhu,Yanbing; Cai,Huinong. |
| Background Carboxyl-functionalized magnetic nanoparticles were synthesized via chemical co-precipitation method and modified with oleic acid which was oxidized by potassium permanganate, and κ-carrageenase from Pseudoalteromonas sp. ASY5 was subsequently immobilized onto them. The immobilization conditions were further optimized, and the characterizations of the immobilized κ-carrageenase were investigated. Results The κ-carrageenase was immobilized onto magnetic iron oxide nanoparticles, and the bonding was verified by Fourier transform infrared spectroscopy. The optimal conditions for κ-carrageenase immobilization were 2.5% (w/v) glutaraldehyde, 13.9 U κ-carrageenase for 20 mg of magnetic nanoparticles, a 2-h... |
| Tipo: Journal article |
Palavras-chave: Carrageenase; Characterization; Immobilization; Nanoparticles. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100001 |
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| I., Manta H.; D., James D.; I., Awu J.. |
| Green house effect and ozone layer depletion due continuous environmental pollution from fossil fuel has speed up the quest for search of alternative energy fuel. In recent year, biofouls has attracted much attention as a reliable substitute to fossil fuel. The objective of this study was to evaluate and characterize biofuel produced from Egusi vegetable oil by applying standard method of transesterification. This study was carried out at Processing and Storage Engineering Departmental (PSED) laboratory of National Centre for Agricultural Mechanization (NCAM), Ilorin Kwara State, Nigeria. The results of the proximate characterization of the produced Egusi vegetable oil shows crude protein, crude fibre, ether extract, moisture content, ash content and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Egusi Vegetable Oil; Characterization; Transesterification; Methyl Esters; Biofuel. |
| Ano: 2021 |
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/6205 |
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| Registros recuperados: 101 | |
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